Rapid preparation of tritium-labeled bile acids by enolic exchange on basic alumina containing tritiated water
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چکیده
منابع مشابه
Tritium labeling of antisense oligonucleotides by exchange with tritiated water.
We describe a simple, efficient, procedure for labeling oligonucleotides to high specific activity (< 1 x 10(8) cpm/mumol) by hydrogen exchange with tritiated water at the C8 positions of purines in the presence of beta-mercaptoethanol, an effective radical scavenger. Approximately 90% of the starting material is recovered as intact, labeled oligonucleotide. The radiolabeled compounds are stabl...
متن کاملHydrolysis products of nucleic acids labeled with tritium; preparation by biosynthesis.
Compounds labeled with Cl4 and N16 have proved useful in studies of nucleic acid metabolism. Since tritium, the radioactive hydrogen isotope of atomic weight 3, is now available in relatively high specific activities, it was decided to explore the usefulness of this isotope in such studies. Inasmuch as there are no data concerning the corresponding use of deuterium, it was first considered nece...
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Alumina nanorods were prepared from chromium-containing alumina sludge, and the effects of doping elements, such as Cr, Fe, and Mg, were researched. The results show that the crystal transformation of alumina is restricted by the doped Cr and facilitated by the doped Fe and Mg, which is transformed from θ-Al2O3 to α-Al2O3 in the calcination process. Meanwhile, the crystal transformation of alum...
متن کاملTritium incorporation in rats chronically exposed to tritiated food or tritiated water for three successive generations.
Wistar rats have continuously ingested tritiated food (48.1 kBq/g) or tritiated water (37.0 kBq/ml) from three weeks before mating of P0 through delivery of the F3 generation. An analysis of tissues at various ages during treatment shows that: (1) tritium incorporation into tissues of rats life-time exposed to tritiated food was on the average 3.53=0.38 times higher than after similar exposure ...
متن کاملTritioboration and synthesis of tritium-labeled polyunsaturated fatty acids.
Methyl esters of polyunsaturated fatty acids labeled with tritium were prepared by partial stereoselective reduction of the corresponding acetylenic esters with tritiated disiamylborane, followed by protonolysis with tritiated acetic acid. The labeling was strictly specific, and the tritium atoms were located only at the carbon atoms forming the unsaturated bond(s). Synthesis of some tritiated ...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1968
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)42682-3